Procedures methods of Using Trace Metals Analyser (PDV6000 pluS) as requested by...

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Metals Analyser The Proper Procedures By: CHEW A.W.

Initial work Reference electrode preparation

1. 
   
   
   
   First-Removed thewhite cap from the reference electrode (White Colour top).
   Secondly soak the reference electrode frit by placing the electrode in to a clean analysis cup containing 10 ml of distilled water or 1M KCL (reference electrolyte) for at least 1 hour, preferably overnight.




2. 
   
   
   
   Ensure the electrode rod is dark brown to black colour or else replating is needed. (mostly needed) Replater will follow back the replating process in the reference book page 50.




3. 
   
   
   
   After the frit has been hydrated, fill the reference electrode with fresh reference electrolyte.
   
   BEFORE each DAY’S work
   Inspection is needed before go to preparation and setup of reagents and samples.
   1. Electrode inspection and preparation. (DO NOT connect the equipment to electricity while doing these following step—because open circuit will occur and damage the equipment)-Expanive you know!!
   

a) Removed all caps (red, blue and white) from the three electrodes.

b)Always ensure the reference electrode (White top) is full of reference electrolyte.

c) Ensure no bubbles in the electrolyte.

d) For the glassy carbon working electrode (blue top) make sure the tip is shiny balck colour, no cracks,no hole or dirt. Rinse it in distilled water and polishing it with supplied polishing kit. ( recommended that the glassy carbon be dipped for 60 seconds in 2M sodium Hydroxide solution (NaOH) and rinse with deionized water.

e)Counter electrode reuired no preparation (No need).

f) Take off the cap from the electrode holder and insert the three electrodes into the corresponding colour by attaching with correct connectors. (DO NOT TOUCH THE

ELECTRODE SURFACE!)

2 Then only come to Instrument setup. (General knowledge – I teach you already or refer to the CD short clips))

3 Sample Preparation ( IMPORTANT!!)

a) Raw sample need electrolyte to ensure efficient conductivity ( more like plating process application)

b) dilution if for stand alone case is in ratio of 1:1. ( maximum fill in cup analysis is 20ml++)

c)At this step: Please refer to the application notes in the white folder file which I kept. (Because in it contains different metals application and different dilution for it)

d) example: 10 mL raw sample + 10 mL electrolyte in analysis cup.

4 Standard Preparation

a)When using PDV 6000 plus,is best chose standard concentration that is nearest to the expected concentration range. (above or below the target limit)

b) Remind ( very helpful for use please remember 100ppb = 0.1 ppm)

c) Please refer to this I test it and more accurate: (also refer to the papers application in folder)

I. Required standard concentration: 10 ppb; Electrolyte volume will be: 20 ml; 20ppm stock standard volume need is : 10 µL
II. Required standard concentration: 50 ppb; Electrolyte volume will be: 20 ml; 20ppm stock standard volume need is : 50 µL
III. Required standard concentration: 200 ppb; Electrolyte volume will be: 20 ml; 20ppm stock standard volume need is : 200 µL
IV. Required standard concentration: 800 ppb; Electrolyte volume will be: 19 ml; 20ppm stock standard volume need is : 800 µL
V. Required standard concentration: 2000 ppb; Electrolyte volume will be: 18 ml; 20ppm stock standard volume need is : 2 µL
VI. Required standard concentration: 8000 ppb; Electrolyte volume will be: 12 ml; 20ppm stock standard volume need is : 8 µL

Noted that all of the standard solution be used is only 10 times, after 10 times must prepare a new standard or else YOUR RESULTS will be not accurate (differ widely from expectation- I was shocked when 1st time use as I am the 1st to used it in whole University Science Malaysia, the testing took me months to get it right)

Then follow as below steps to run the detection of metals ion concentration.

FOR THE VAS/ STANDALONE CASE PDV 6000 plus (meaning only the analyzer without connecting to electricity source by only using batteries)

FIVE MAIN STEPS: (For field work)
1) Sample preparation
2) Electrodes preparation
3) Running blank
4) Analyzed standard of known concentration
5) Analyzed samples

These procedures is also similar to the VAS when PDV 6000 plus is attached to your laptop or PC.